Acid Cellulase for Bio-polishing(POWDER)

SUKACell-P1000Acid Cellulase for Bio-polishing INTRODUCTION Cellulose is an unbranched glucose polymer composed of anhydro-D-glucose units linked by 1, 4-b-D-glucoside bonds. These glycosidic bonds can be hydrolysed by cellulolytic enzymes. The native structure of the cellulose is composed of crystalline and less organized amorphous regions. SUKACell-P1000 is an enzyme preparation obtained from the submerged fermentation of a high cellulase-producing microbial strain. It is widely used in textile and garment stone wash industries. WORKING MECHANISM The 1, 4-b-D-glucosidic linkages in cellulose, lichenin and cereal b-D glucans are hydrolyzed to release free glucose units through the successive action of cellulase enzymes. The less organized amorphous region at the center of the cellulose chain is initially attacked by endoglucanase by random cutting of the b-1, 4 glycosidic linkages within the chain thereby producing cello-oligosaccharides. Cellobiohydrolases I and II act on the cello-oligosaccharides from the reducing end and non-reducing end of the cello-oligosaccharides to release cellobiose. b- glucosidase act on the released cellobiose to produce b–D glucose units. PROPERTIES SUKACell-P1000 will function from a pH of 4.5 to 5.5; with pH 4.8 as an optimum. It will function from 45°C to 60°C and optimum temperature is 55°C.Appearance: Cream to white powder (Note that color does not affect or reflect activity.)Odor: Slight fermentation odorpH (1% soln) 3.5-5.5SPECIFICATION SUKACell-P1000(activity is 4000 U/g)High activity product is available upon request of our customers.ENZYME ACTIVITY UNIT DEFINITION Reducing sugar method At a pH of 4. 8 and temperature of 50 °C, to produce the amount of reducing sugar equivalent to 1 mg glucose, it needs l g solid enzyme (or 1mL liquid enzyme) and 1 h hydrolyze sodium carboxymethyl cellulose substrate, as 1 enzyme activity unit, state as u/g (or u/mL). CMCA – DNS for short. EXECUTION STANDARD Light industry standard of the People's Republic of China, QB 2583 2003 USAGE SUKACell-P1000 - is a kind of acidic solid cellulase, used for biofinishing, significantly soften 100% cotton fabrics, both woven and knits.SUKACell-P1000 – provide soft touch except used for jean leaven dye processing.SUKACell-P1000 - can obviously improve clothing surface magnitude.OPERATION & DOSAGE OPERATIONDOSAGEBiofinishingStonewashingDesize thoroughlyDesize thoroughlyDesize thoroughlyDrain liquor then fill machine to liquor ratio of1:10 (jean/ water)1:10 (jean/ water)Add pumice (if required), heat bath to40-60°C50-60°CAdd SUKACell-P1000at the rate of0.25-2.0% owg0.25-0.75% owgAdjust pH to4.5-5.5,with pH 4.8 as an optimum4.5-5.5,with pH 4.8 as an optimumProcess time20-45 minutes(depending on desired effect)30-45 minutes(depending on desired effect)after drainageAdd water and softeningAdd water and softeningNOTE:Some denim fabrics of lower quality may have severe backstaining problems that can be overcome with our ES resist. Use soda ash 1-3 grams and resist ES 1-2 grams (water ratio) to wash out ferment remaining residue, after that you can adopt our consistent lubricant/ film/ organic silicon oil. The information presented is believed to be accurate. However, said information and products are offered without warranty or guarantee. Sales commitment should pay legal responsibility. We recommend that the prospective user determine the suitability of our materials and suggestions before adopting them on a commercial scale. PACKAGING SUKACell-P1000 is packaged in 25 kg plastic drum for the solid form.Alternative packaging is available upon request for smaller or larger volumes.STORAGE Store in a cool, dry place and away from direct sunlight. SUKACell-P1000 maintains its activity for considerably longer time when stored at lower temperatures (below 25°C). Do not let freeze. HANDLING PRECAUTIONS SUKACell-P1000 is non-toxic and biodegradable. However, unnecessary contact with the product should be avoided. Long term exposure to protein based products such as SUKACell-P1000 may sensitize certain individuals to the product. Wash hands with warm soapy water after handling. Keep out of reach of children. TECHNICAL SERVICE SUKAHAN (Weifang) Bio-Technology Co., Ltd will assist customers with the use of our products in applications development.